EFFECTS OF FEEDING POLYUNSATURATED OR SATURATED FATTY ACIDS TO PRE-RUMINATING CATTLE ON DIFFERENTIATION AND GROWTH OF BOVINE PREADIPOCYTES FROM INTRAMUSCULAR AND SUBCUTANEOUS FAT DEPOTS
Loucks, William Isaac. (2016). EFFECTS OF FEEDING POLYUNSATURATED OR SATURATED FATTY ACIDS TO PRE-RUMINATING CATTLE ON DIFFERENTIATION AND GROWTH OF BOVINE PREADIPOCYTES FROM INTRAMUSCULAR AND SUBCUTANEOUS FAT DEPOTS. Theses and Dissertations Collection, University of Idaho Library Digital Collections. https://www.lib.uidaho.edu/digital/etd/items/loucks_idaho_0089n_11020.html
- Title:
- EFFECTS OF FEEDING POLYUNSATURATED OR SATURATED FATTY ACIDS TO PRE-RUMINATING CATTLE ON DIFFERENTIATION AND GROWTH OF BOVINE PREADIPOCYTES FROM INTRAMUSCULAR AND SUBCUTANEOUS FAT DEPOTS
- Author:
- Loucks, William Isaac
- Date:
- 2016
- Keywords:
- alpha linolenic acid conjugated linoleic acid preadipocytes
- Program:
- Animal and Veterinary Science
- Subject Category:
- Animal sciences
- Abstract:
-
Twelve newborn Holstein bull calves were divided into cohort groups and fed (1. PUFA-2:1 flaxseed oil:CLA or 2. SFA – palm oil) in 2L milk twice daily for 50 d to determine the effect on preadipocyte development in pre-ruminating cattle. Fatty acid profiles, cell growth, morphological differentiation, and adipose tissue gene expression were measured. In PUFA fed calves, CLA was greater in SC (P<0.01), PR (P<0.001), calf serum (P<0.0002) and trended in IM (P<0.06). No significant level of ALA was present in any tissue samples; however, serum from the PUFA treatment had 20-fold higher ALA. No treatment effect was seen on relative gene abundance. Subcutaneous clonal preadipocytes grew faster with PUFA treatment calf serum (P=0.03), and isolated PUFA treatment SC S-V cells differentiated more than intramuscular preadipocytes (P=0.01). Feeding specific FAs affects tissue and serum FA profiles and aspects of adipocyte development.
- Description:
- masters, M.S., Animal and Veterinary Science -- University of Idaho - College of Graduate Studies, 2016
- Major Professor:
- Doumit, Matthew E
- Committee:
- Murdoch, Gordon K; Balemba, Onesmo
- Defense Date:
- 2016
- Identifier:
- Loucks_idaho_0089N_11020
- Type:
- Text
- Format Original:
- Format:
- application/pdf
- Rights:
- In Copyright - Educational Use Permitted. For more information, please contact University of Idaho Library Special Collections and Archives Department at libspec@uidaho.edu.
- Standardized Rights:
- http://rightsstatements.org/vocab/InC-EDU/1.0/